A novel gene induced during hypersensitive reaction (HIR) in wheat was identified using in silico cloning and RAMEN MUSHROOM designated as TaHIR2.The TaHIR2 gene was deduced to encode a 284-amino acid protein, whose molecular mass and isoelectric point (pI) were 31.05 kD and 5.18, respectively.
Amino acid sequence analysis demonstrated the presence of stomatins, prohibitin, flotillins, HflK/C (SPFH) domain and prohibitin homologue for the TaHIR2 protein.Phylogenetic analysis of 13 HIR genes from different monocots indicated that TaHIR2 was highly homologous to HvHIR2.Transient expression analysis using particle-mediated bombardment showed that the TaHIR2 fusion protein was located in the onion epidermal cells.Quantitative RT-PCR analyses revealed that TaHIR2 transcripts were significantly accumulated in adult wheat leaves with maximum induction at 18 h post inoculation with the stripe 6.13/6BG rust, whereas slightly up-regulation could also be observed in the compatible reaction at the seedling stage.
These results suggest that TaHIR2 may play an active role in wheat defense against stripe rust.